Rice , , Springer Protocols. Associated articles This version , Stephen Thompson, References Gallagher, S. Protocols Immunol. Thompson, S. Cell Sci. Stern, P. Hubbard, A. Cell Biol. Acta , — Blackmore, M. Metastasis 8 , — Laemmli, U. Nature Lond. Cancer Lett. Laskey, R. FEBS Lett. TidyBlot, in contrast to conventional secondary antibodies, only binds to the native antibody used during the western blotting procedure and therefore enables the western blot detection of IP samples without interference from IgG heavy and light chains.
Please note that TidyBlot is HRP conjugated and that the western blot bands red and protein standards green have been pseudocolored. Bio-Rad customers are successfully using TidyBlot to obtain high quality, publishable western blotting data.
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Third-Party Cookies being blocked. Please Note. These techniques take advantage of the high specificity and tight binding that antibodies provide to separate the desired proteins out from the background of all the other cellular proteins present. Immunoblotting also serves to establish or verify the identity of proteins isolated in immunoprecipitation.
A vast number of other questions can also be addressed by these two related techniques. Unable to display preview. Download preview PDF.
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